云南红豆杉天然种群遗传多样性研究

STUDY ON GENETIC DIVERSITY OF NATURAL POPULATIONS OF TAXUS YUNNANENSIS

采用垂直板式聚丙烯酰胺凝胶电泳 ,检测了云南红豆杉 (Taxusyunnanensis)雌配子体的 5种等位酶 ,并以 10个基因位点编码的 5个酶系统 ,分析了滇西北 3个云南红豆杉天然种群的遗传多样性与遗传分化。结果表明 ,群体的多态位点比例是 0 77;群体的平均杂合性观察值是 0 30 2 ;预期值为 0 332 0 ;每个基因位点发现的等位基因数是 2 0 5 ;有效等位基因平均数是 1 46 6。对 10个基因位点的基因多样性测定表明 ,种群间的分化占 14 6 6 % ,总的基因多样性约 85 %产生于种群内。群体间的平均遗传距离是 0

Vertical slab polyacrylamide gel electrophoresis was used to examine inheritance of five isoenzymes in megagametophytes of \%Taxus Yunnanensis.\%Genetic diversity and genetic differentiation in three natural populations of \%Taxus Yunnanensis\% in Northwestern Yunnan were analyzed at ten loci coding for five enzyme systems.The percentage of loci polymorphism was 0 77.The average observed heterozygosity of all populations was 0 302,and the average expected heterozygosity was 0 320.The average number of alleles detected per locus was 2 05.The effective number of alleles per locus was 1 466.Measurement of gene diversity for ten loci showed that interpopulation genetic differentiation was 14 66%,and about 85% of the total gene diversity resided within population.The mean genetic distance was 0 118.