性激素水平及平衡对大鼠血管内皮细胞增殖的调节作用及意义

Regulating effect and significance of sex hormone levels and balance on the proliferation of rat vascular endothelial cells

目的 探讨雌、雄及孕激素分别对雌、雄性大鼠肺血管内皮细胞 (VEC)增殖的影响。方法 采用组织贴块法培养大鼠肺血管内皮细胞 ,应用噻唑蓝 (MTT)比色法检测大鼠VEC的增殖情况。结果 (1) 3× 10 -8mol/L、3×10 -7mol/L 17 β雌二醇 (E2 )可促进雌鼠肺VEC的增殖 (P <0 .0 1) ;3× 10 -9mol/L、3× 10 -8mol/L、3× 10 -7mol/L 17 βE2 均能促进雄鼠VEC的增殖 (P<0 .0 5 ) ,各组间无明显差异。雌激素受体拮抗剂他莫昔芬可阻断 17 βE2 上述促增殖作用。 (2 ) 3× 10 -8mol/L、3× 10 -7mol/L睾酮 (T)均可明显促进雄鼠VEC的增殖(P <0 .0 5 ) ,但对雌鼠VEC增殖无促进作用。 (3)E2 /孕激素 (P) =3/ 10时能促进雌鼠VEC的增殖 (P<0 .0 5 )。(4)E2 /T =1亦可促进雌鼠VEC增殖 (P<0 .0 5 )。结论 雌激素可促进雌、雄性大鼠VEC的增殖 ,其促增殖作用无性别差异 ,且通过雌激素受体 (ER)介导。雄激素仅可促进雄性大鼠VEC增殖 ,单纯孕激素对雌鼠VEC增殖无明显影响。E2 /T、E2

Objective To investigate the effects of estrogen,progesterone(P) and testosterone on the proliferation of vascular endothelial cells(VEC) of female and male rats respectively.Methods Rat lung vascular endothelial cells were cultured according to the blocks explanting method.MTT assay was used to measure the proliferation of VEC.Results (1)17 β estradiol(E 2)at 3×10 -8 mol/L and 3×10 -7 mol/L accelerated the proliferation of female rat VEC(P<0.01).17 β estradiol at 3×10 -9 mol/L,3×10 -8 mol/L and 3×10 -7 mol/L accelerated the proliferation of male rat VEC(P<0.05).Tamoxifen(estrogen receptor antagonist) could block the effect of estrogen on the proliferation of VEC.(2)Testosterone(T) at 3×10 -8 mol/L and 3×10 -7 mol/L significantly increased the proliferation of male rat VEC(P<0.05),but had no effect on female rat VEC.(3)When the ratio of E 2 to P was 3 to 10,the proliferation of female rat VEC was accelerated(P<0.05).(4)When the ratio of E 2 to T was 1 to 1,the proliferation of female rat VEC was also accelerated(P<0.05).E 2 and T at a ratio of 1 to 100 had no effect on female rat VEC.Conclusions Estrogen can accelerate the proliferation of female and male rat VEC through mediation by estrogen receptor.Testosterone only increases the proliferation of male rat VEC,and progesterone alone has no effect on female rat VEC.The balance of the ratios of E 2 to T(E 2/T) and E 2 to P(E 2/P) may play an important role in the proliferation of female rat VEC.