再灌注致大鼠心肌线粒体损伤及益心康胶囊的保护作用

Injurious effect of ischemic reperfusion and the protective effect of Yi-Xin-Kang capsule on rat myocardial mitochondria

目的 观察再灌注对心肌线粒体的损伤 ,评价中药复方———益心康胶囊 (H30 3)的保护作用。方法 利用离体大鼠全心停灌 /再灌 (I/R)模型 ,测定心肌线粒体结构和功能的变化。结果 I/R可致线粒体结构和功能损伤 ,H30 3预先灌注给药可明显减轻心肌脂质过氧化程度 ,抑制磷脂酶A2 活性 ,抑制线粒体磷脂降解和游离脂肪酸生成 ,改善线粒体膜脂流动性。此外对呼吸功能及Ca2 + ATPase活性也具有明显的保护作用。结论 H30

Objective To observe the injury of mitochondria induced by reperfusion and to evaluate the protective effect of Yi Xin Kang capsule(H3O3),a traditional Chinese medicinal prescription (H303).Method Using the model of global ischemia/reperfusion (I/R) of the rat heart,the changes of the structure and function of myocardial mitochondria were measured.Results The results demonstrated that I/R caused severe injury of the structure and function of myocardial mitochondria.Pretreatment with H303 by perfusion showed protective effects through decreasing lipid peroxidation of myocardium,the activity of phospholipase A 2 (PLA 2) and the contents of phospholipid (PL) and free fat acid (FFA) in myocardial mitochondria and improving membrane lipid fluidity (LFU).In addition,H303 also possessed the effects of protecting the activity of Ca 2+ ATPase and the respiratory function of mitochondria.Conclusion H303 has the significant protective effects on mitochondria injury induced by I/R.