反应条件对深黄被孢霉催化转化十六醇合成不饱和脂肪酸的影响

Effects of Reaction Conditions on Biosynthesis of Fatty Acids from Hexadecanol by Mortierella Isabellina

研究了深黄被孢霉催化转化十六醇合成不饱和脂肪酸过程中反应条件对底物转化率及产物选择性的影响 .结果表明 ,亚油酸的选择性随十六醇与酵母膏比例 (碳氮比 )的增大而升高 ,亚麻酸的选择性随碳氮比的增大而降低 ,油脂的选择性和油脂的产率随碳氮比的变化规律相似 .十六醇浓度为 0 5 %时 ,油脂的选择性、产率及醇的转化率最高 ,亚油酸和亚麻酸的选择性也最高 .油脂的选择性和产率在 pH =7时较高 ,而醇的转化率随pH升高而降低 ,亚油酸和亚麻酸的选择性在 pH =6时分别达到最大值 .油脂的选择性、产率和醇的转化率在 2 3～ 2 8℃达到较高水平 ,亚油酸和亚麻酸的选择性在 2 3℃达到最大值 .油酸的选择性对油脂的选择性有重要影响 。

The effects of reaction conditions on catalytic performance of Mortierella isabellina in the process of converting hexadecanol. to unsaturated fatty acids were investigated. The selectivity for linoleic acid increases with increasing ratio of hexadecanol to yeast extract in the culture broth, while the selectivity for linolenic acid and the conversion of hexadecanol decrease. The conversion of hexadecanol and selectivity for lipids, linoleic acid, and linolenic acid reach their maximum values, respectively, with the hexadecanol concentration of 0.5%. The conversion of hexadecanol decreases with the increase of PH value. Lipid selectivity has a maximum at pH = 7, but the selectivity for linoleic acid and linolenic acid, at pH = 6. The incubation temperature range of 23 similar to 28 degreesC is favorable for promoting the hexadecanol conversion and the lipid-selectivity. The highest selectivity for linoleic acid and linolenic acid appears at 23 degreesC.