尿中磺酸化胆汁酸的流动注射固定化酶化学发光法

A Flow Injection Analysis Chemiluminescence Method for Determination Sulfated Bile Acids in Urine by Using an On-line Immobilized Enzyme Reactor

肝胆系统有疾患，会导致尿中磺酸化胆汁酸浓度的增加。根据尿中磺酸化胆汁酸的浓度可以 判断肝脏机能是否正常。基于流动注射分析原理，利用化学发光法和固定化酶反应，建立了一个新的尿 中磺酸化胆汁酸的临床快速分析法。进样量为２０μＬ；分析速度３０样／ｈ；检出限为０．１μｍｏｌ／Ｌ；ＲＳＤ小于 ２．２％；线性范围在０．１～１２μｍｏｌ／ｌ之内。

According to the content of sulfated bile acids (SBAs) in urine the function of hepatobiliary can be judged whether normality. Based on the flow-injction analysis, chemiluminescence and immobilized enzymatic reaction, we have established an accurate and sensitive method for the determination of SBAs in urine. Its sampling frequency is 30 sample/h and relative standard deviation is less than 2.2%. The calibration curve of the method also shows good linearity over a range of 0.1 similar to 12 mu mol/L glycolithocholic acid 3-sulphate as a standard. The approach proposed can be applied to clinic tests of SBA in urine or blood at hospitals.