正相高效液相色谱-手性配位基交换流动相法测定人血清中的甲状腺素对映体

Determination of Thyroxine Enantiomers in Human Plasma with Normal High Performance Liquid Chromatography-Chiral Complex Exchange Mobile Phase

运用正相高效液相色谱 手性配位基交换流动相法 ,通过流动相中添加L 脯氨酸、醋酸铜及三乙胺 ,对人血清中甲状腺素对映体 (D ,L T4)进行了测定。在所建立的色谱条件下 ,D ,L T4异构体得到基线分离。该法用于测定甲状腺机能亢进 (甲亢 )、甲状腺机能减退 (甲低 )患者血清中的D ,L T4的浓度 ,可为临床用药提供理论依据 。

An HPLC method was developed with normal HPLC chiral complex mobile phase for the separation and determination of D and L thyroxine enantiomers (D and L T4) in human plasma The method includes extraction of thyroxine from plasma and separation of thyroxine enantiomers on HPLC silica column with chiral eluent containing L proline, cupric acetate and triethylamine (TEA) The sensitivity of the method was 0 1 mg/L The precisions of inter day and intra day, linearity, extraction recovery, and stability of T4 enantiomers in plasma and in deproteinized plasma were determined for the validation of the method Baseline enantioseparation of the compounds containing D and L T4 was achieved Meantime we determined the concentrations of D T4 and L T4 in plasma of 15 volunteers with euthyroid, hypothyroid and hyperthyroid symptoms. Liquid chromatographic method based on the described procedure was useful for the determination of D and L thyroxine in patient plasma and for pharmacokinetics investigation