摘要
采用强阳离子交换柱分离 ,pH梯度洗脱 ,邻苯二甲醛 (OPA)柱后衍生 ,荧光λex=338nm ,λem=42 5nm检测的高效液相色谱法测定了南瓜粉中的 4 氨基丁酸 (GABA)。若以GABA的峰高Y(μV)对进样质量X(μg)进行线性回归 ,则线性方程为Y =45 6 6X +1396 ,r =0 9998;GABA的平均回收率 (n =3)为 99%。方法稳定、快速、灵敏。
A method for the separation and determination of the 4 aminobutyric acid in pumpkin powder by high performance liquid chromatography (HPLC) with post column derivatization and fluorescence detection is described The operating conditions were cation exchange resin column (30 cm×0 4 cm i d ) with gradient elution of buffer solutions A [19 6 g sodium citrate dihydrate and 1 0 g phenol dissolved in 1 L water, pH(3 15±0 02)] and B [21 0 g sodium nitrate and 1 5 g boric acid dissolved in 1 L water, pH(9 70 ±0 02)] as mobile phase at a flow rate of 0 5 mL/min, and a column temperature of 62 ℃, with detection wavelength λ ex =338 nm, λ em =425 nm The retention time of 4 aminobutyric acid was 34 77 minutes The average recovery was 99% and the coefficient of variation was 1 35% This method is simple, rapid and sensitive
出处
《色谱》
CAS
CSCD
北大核心
2001年第6期532-533,共2页
Chinese Journal of Chromatography
