反相高效液相色谱法测定小鼠血浆及组织中的阿昔洛韦浓度

Determination of Acyclovir in Mouse Plasma and Tissues by Reversed-Phase High Performance Liquid Chromatography

建立了测定小鼠血浆、肝、肾、脾、肺等组织中阿昔洛韦 (ACV)浓度的高效液相色谱法。色谱柱为HypersilODS ,流动相为甲醇 水 冰醋酸 (体积比为 1∶99∶0 5 )混合溶液 ,流速为 1 5mL/min ,检测波长为 2 5 2nm。ACV血浆最低检测浓度为 2 0 μg/L ,各组织最低检测浓度为 5 0ng/g。血浆及组织匀浆中的ACV浓度在 0 1mg/L～ 4mg/L及 0 1μg/g～ 4μg/ g时线性关系良好 (r >0 99)。血浆及肝匀浆中的ACV回收率分别为 97 5 %～ 10 0 0 %和 10 0 0 %～ 10 6 0 % (n =5 )。该法精密度高 ,方便 。

The aim of this study was to establish an high performance liquid chromatographic method for determining acyclovir(ACV) concentration in mouse plasma and tissues A solution of 0 25 mL 60 g/L perchloric acid and 0 25 mL acetonitrile was added into 0 2 mL plasma or 0 2 g tissues to precipitate proteins Following centrifugation, the supernatant obtained was injected into a reversed phase column Operating conditions were Hypersil ODS column(250 mm×4 6 mm i.d.,5 μm), methanol water acetic acid(1∶99∶0 5, volume ratio) solution as mobile phase at a flow rate of 1 5 mL/min, UV detection at 252 nm The detection limit of ACV concentration in plasma was 20 μg/L and that in tissues was 50 ng/g The standard curves for ACV were linear in plasma and homogenate of tissues ( r >0 99) The precision of the method was good and the recoveries of ACV were higher than 97 5%. So this method is rapid, accurate and convenient for determination of ACV concentrations in plasma and tissues