摘要
The effects of extraction time,alkaline concentration,and the addition of cartilage on the extraction of mucopolysaccharides from shark fin cartilage digested with NaOH were investigated.The main components of mucopolysaccharides during the degrading process were analyzed by cellulose polyacetate strip electrophoresis and agarose gel electrophoresis.The results showed that the extraction of mucopolysaccharides was increased with the increase of extraction time,alkaline concentration and the addition of cartilage.According to the different electrophoretic migrations,the preparative mucopolysaccharides composed of different polysaccharides with electronegativity can be divided into two parts,the chondroitin sulfate with higher migration and the other with lower migration.The lower part is unstable and easily degraded with alkaline solution.
The effects of extraction time,alkaline concentration,and the addition of cartilage on the extraction of mucopolysaccharides from shark fin cartilage digested with NaOH were investigated.The main components of mucopolysaccharides during the degrading process were analyzed by cellulose polyacetate strip electrophoresis and agarose gel electrophoresis.The results showed that the extraction of mucopolysaccharides was increased with the increase of extraction time,alkaline concentration and the addition of cartilage.According to the different electrophoretic migrations,the preparative mucopolysaccharides composed of different polysaccharides with electronegativity can be divided into two parts,the chondroitin sulfate with higher migration and the other with lower migration.The lower part is unstable and easily degraded with alkaline solution.
出处
《化工学报》
EI
CAS
CSCD
北大核心
2001年第8期726-729,共4页
CIESC Journal
基金
国家教育部科学技术重点项目 (教财司 [1999] 2 84)
国家自然科学基金资助项目 (No .2 0 0 0 60 0 5 )&&
关键词
粘多糖
鲨鳍软骨
提取
电泳
分析
碱降解法
mucopolysaccharide,shark fin cartilage,extraction,degradation,electrophoresis
