过氧化氢酶与Cu(Ⅱ)络合的ESR谱及活性分析

Analysis on enzyme activity and ESR spectra of Cu(Ⅱ)-catlase

用Cu(Ⅱ )对过氧化氢酶 (CAT)进行修饰 ,制备了Cu(Ⅱ )络合的CAT ,称为Cu(Ⅱ )CAT .Cu(Ⅱ )CAT除了具有CAT的活性外 ,还有SOD样活性 .Cu(Ⅱ )CAT可以抵抗O2

In this paper,Cu(Ⅱ)modified CAT(Catalase)was prepared,and temporarily named it Cu(Ⅱ)CAT.It had CAT activity (it was about 98.69% of native CAT)and some SOD-like activity(0.976 U/uL,Cu(Ⅱ)CAT being 0.001 mol/L).It's studied with oxygen radical (Xanthine Oxidase/Xanthine,XO/X)that the experimental damage of Cu(Ⅱ)CAT.The results after damaging 20 minutes showed:the amino acid residues of Cu(Ⅱ)CAT protein was not oxidated (carbony1 contain was only 0.014 umol/mg·)and apparent K m (46.8 mmol),α helix contain(22.97%)had no significant change as comparison with native CAT,and had significant change as comparison whth CAT treated by the oxygen radical.These results suggested that Cu(Ⅱ)CAT can withstand the oxygen radical damage.