间接原子吸收法测定硫酸庆大霉素

Study on determination of gentamycini by indirect atomic absorption spectorscopy

建立了在镍离子存在下测定硫酸庆大霉素的间接原子吸收法。硫酸庆大霉素与二硫化碳反应 ,生成不溶于碱液的二烷基二硫代氨基甲酸镍沉淀 ,离心分离后以AAS法测定沉淀中镍的量来间接确定硫酸庆大霉素的含量 。

A method of determination of gentamycini indirectly by atomic absorption spectroscopy was described. Gentamycini reacted with CS 2 in Ni 2+ medium and formed a precipitate of R 2N(CSS) 2 Ni or (R 2NH-CSS) 2Ni. After separation of the precipitate from the solution, the amount of Ni was determined by AAS, and the amount of gentamycini was determined indirectly. The method was used to determine gentamycini in samples with satisfactory results.