长春花碱在牛体外受精胚中染色体标本处理及性别分析

SEXING AND VINBLASSTINE TREATMENT FOR CHROMOSOME PREPARATION IN BOVINE EMBRYOS DERIVED FROM IN VITRO FERTILIZATION

长春花碱是一种常用的细胞分裂阻止剂 ,在细胞遗传学分析及体外受精胚的性别鉴定等方面有着重要意义。该研究对牛体外受精胚的第一卵裂期染色体标本制作及性比进行了分析。废弃牛卵巢内卵子经体外成熟培养 ,体外受精 2 0h后 ,在培养基中添加 10 0ng/ml长春花碱处理 8h或 10h。结果表明 ,用长春花碱处理 8h的胚胎标本大部分含有可分析的中期分裂像并可进行性别判断 ,明显高于 10h处理的胚胎标本 (P <0 .0 5 )。性比分析表明 ,牛体外受精的胚胎的雄性发生率略高于雌性发生率。

The required concentration and time duration of vinblastine sulfate treatment were determined for successful cytogenetic diagnosis, such as normality and sex in bovine embryos derived from in vitro fertilization. Twenty hours after insemination, the embryos were placed on a medium containing 100 ng/ml vinblastine sulfate and cultured for 8 and 10 hours. The rate of the embryos with mitotic metaphase figures and sex ratio in the medium cultured for 8 hours was significantly higher than that cultured for 10 hours ( P <0.05). A sex ratio with a slight male advantage was found in the present study.