小鹅瘟病原分离鉴定及高免卵黄抗体的研制

Isolation and identification of gosling plague antigens and preparation of highly immunized egg yolk antibodies

无菌采取怀疑患小鹅瘟雏鹅的脑、肝、脾、肾等组织样本 ,健康鹅胚尿囊腔接种分离病毒 ,经雏鹅保护试验及鹅胚内中和试验鉴定 ,确诊病雏鹅的病原为小鹅瘟病毒 .应用所分离的病毒制备高免卵黄抗体 ,经效力检验 ,取得对小鹅瘟攻毒保护10 0

Tissue samples ware aseptically collected from the brains,livers,spleens and kidneys of goslings suspected of having plague,and allantoic cavities in viable goose embryos were vaccinated with isolated viruses.Gosling protection and in embryo nuetralization trials confirmed that the antigen causing goslings to be ill was a gosling plague virus.Highly immunized egg yolk antibodies prepared with isolated viruses could yield 100% protection against gosling plague.