摘要
用间接ELISA检测伪狂犬病病毒 (PRV闽A株 )自制的油乳剂灭活苗、氢氧化铝胶灭活苗免疫山羊和犊牛后的抗体效价 ,并以市场购买的基因缺失油乳剂灭活苗作对照 ,依据山羊抗体的ELISA效价找出抗体消长规律 .采集犊牛、山羊血清用中和试验测抗体 .试验结果表明 ,(1)首次免疫后抗体上升幅度低 ,仅 2~ 3个滴度 ,且维持时间短 ,仅为 2周 ;二免后抗体上升可高达 12个滴度 ,且维持时间长达 4个月 .(2 )试验组油乳剂灭活苗免疫效果优于氢氧化铝胶灭活苗 .(3)
Studies were conducted to minitor the serum antibody of young cows and young goats after being immunized with three kinds of PRV inactive vaccines,self made oil emulsion assistant inactive vaccine,Al(OH) 3 assistant inactive vaccine and pseudorabies virus gene defected inactive vaccine bought on the market.The serum samples from goats are monitored with indirect ELISA.While the serum samples from young cows are monitored with neutralization experiment and are compared with goats antibody level monitored with the same method.The result showed that (1) after the first immunization,the antibody level increased only by 2~3 titers for only 2 weeks.After the second immunization,the antibody level increased highly 12 titers for 4 months.(2) The effect of oil emulsion assistant inactive vaccine is better than that of the Al(OH) 3 assistant vaccine.(3) The contrast of the antibody level of goats and cows showed that they had the similar antibody level and dynamic regularity.
出处
《河南农业大学学报》
CAS
CSCD
2001年第3期275-277,共3页
Journal of Henan Agricultural University
基金
"九五"国家重中之重科技攻关计划项目 ( 96 0 0 30 30 8)
关键词
牛
山羊
伪狂犬病
灭活苗
抗体消长规律
酶联免疫吸附试验
cow
goat
pseudorabies
inactive vaccine
antibody dynamic regularity
enzyme linked immunosorbent assay