高密度培养基因工程大肠杆菌生产α-乙酰乳酸脱羧酶

Produce of α-Acetolactate Decarboxylase in Engineering Escherichia coli by High Density Culture

在 30 0 0 L 发酵罐中 ,利用分批补料培养技术高密度培养含分泌型重组质粒 p ETGAU- 10的大肠杆菌TG1,生产α-乙酰乳酸脱羧酶。通过控制发酵条件 ,发酵液的细胞密度 (OD60 0 )可达 30 .5 ,且胞外α-乙酰乳酸脱羧酶活力最高达 890 U .ml- 1 ,达到较为理想的工业化生产的要求。

Escherichia coli TG1,which contained secreted recombinant plasmid pETGAU 10,was cultured to a high density to produce α ALDC by the fed batch culture in 3 000 L fermentor In control of the fermentative conditions,the cell density can reach as high as 30 5 of OD 600 and the highest extracellular activity of α ALDC reached 890 U·ml -1 ,which meet the demand for the ideal industrial production