反应停对慢性肝炎患者外周血单核细胞肿瘤坏死因子α的抑制作用

Inhibition of Thalidomide on Tumor Necrosis Factor α and Other Cytokins Produce d by Human Peripheral Blood Mononuclear Cells from Patients with Chronic Hepatitis

目的 观察反应停对慢性肝炎的治疗作用。方法 用淋巴细胞份离液从 13例慢性肝炎患者的外周血中分离单核细胞 ( peripheralbloodmononuclearcells,PBMCs)进行体外培养 ,用酶联免疫吸附法、免疫组化法观察反应停对PBMCs肿瘤坏死因子α(TNFα)和其他细胞因子产生的影响。结果 反应停可抑制PBMCsTNFα和其他细胞因子的产生。结论 反应停对慢性肝炎的治疗有重要的意义。

ObjectiveTo observe the treatment effects of Thali domide on the patients with chronic hepatitis.MethodsPeripheral blood mononuclear cells (PBMCs) were isolated from 13 patients with chronic hepa titis by density gradient centrifugtion, then cultured in vitro, the effects of Thalidomide on the syntheses and secretion of TNF-α, IL-1α,IL-6, IL-8 were st udied with enzyme-linked immunosorbent assay and immunohistochemical method.ResultsThalidomide could reduce the syntheses and secretions of TNF- αP<0.05) and other cytokines, including IL-1α, IL-6 and IL-8 induced by L PS (P<0.05)ConclusionsThalidomide or its analogues could be as a new therapeutic drug to treating chronic hepatitis.