急性呼吸道感染多病原体的临床病原学早期诊断

The Study on the Detection of Pathogens for the Acute Respiratory Infection in the Early Diagnosis of Clinical Etiology

目的 研究实用的急性呼吸道感染 (ARI)病原学诊断方法 ,供临床应用。方法 ARI病人 10 8例为实验组和健康人 4 0例为对照组 ,采集咽部分泌物为实验标本 ,用聚合酶链反应 (PCR)技术检测腺病毒 (ADV)、柯萨奇病毒 (COX)、呼吸道合胞病毒 (RSV)、肺炎衣原体 (CP)和肺炎支原体 (MP)。结果 ARI组的病原体检出率分别为 :ADV 10 2 %、COX 17 6%、RSV 2 8 7%、CP 33 3%和MP 13 9%。ARI组与对照组的PCR结果有显著差异(P <0 0 1)。结论 实验显示 ,PCR技术是一种很实用的ARI临床病原学诊断方法 ,ADV、COX、RSV、CP和MP是重要的导致ARI的病原体。

Objective To study the practical methods for the diagnosis of clinical etiology of acute respiratory infection(ARI).Methods 108 patients with ARI were the expermental group and forty healthy peoples were the control group.The specimens from throaty excretions were collected in the both groups.Adenovirus(ADV),coxsackie virus(COX),respirator syncytial virus(RSV),chlamidia pneumonia(CP) and mycoplasma pneumonia(MP) in the specimens were detected with polymerase chain reaction(PCR).Results The detected positive rates were 10 2% for ADV,17 6% for COX,28 7% for RSV,33 3% for CP and 13 9% for MP respectively in ARI.There was significantly difference between the ARI group and the healthy control group(P<0 01).Conclusions PCR is a better useful detected method in the clinical etiologic detection of ARI.ADV,COX,RSV,CP and MP are important pathogens to cause ARI.