贵阳市淋球菌耐药质粒及其与淋病流行的关系

The Gonococcol R Plasmids and Its Relationship with Gonorrhea Epidemic in Guiyang City

目的 为了了解贵阳市淋球菌耐药质粒的大致范围及淋球菌耐药质粒在细菌间的流向。方法 采用改良的Jo .Annes法对 2 0株淋球菌临床分离株进行质粒提取 ,测定其分子量的大小 ;从中筛选出产青霉素酶淋球菌菌株(PPNG) ,分别与大肠杆菌和淋球菌青霉素敏感菌株进行接合和转化试验。结果 2 0株淋球菌临床分离株检出 7株PP NG菌株 ,淋球菌 4.5Md质粒的携带率为 40 % ,3.7Md质粒的携带率为 2 5 %。 3株带有 2 4.5Md质粒的PPNG菌株 ,其耐药 (R)质粒均可通过接合而传递给大肠杆菌和淋球菌敏感菌株 ;2株PPNG菌株可通过转化将R质粒传递给大肠杆菌和淋球菌敏感菌株。结论 贵阳市流行的淋球菌中有 2种耐药质粒存在 ,以 4.5Md质粒为主 ;PPNG菌株可通过接合和转化的传递方式 ,将耐药质粒在淋球菌青霉素敏感菌株和大肠杆菌中传递 。

Objective To learn more about the approximate range of drug resistance plasmids in Guiyang city,and gonorrhoeae R plasmid's flow direction in the bacterias.Methods 20 gonocous clinical isolates strains's plasmids were extracted by means of modified Jo.Annes test.PPNG strains which isolated from 20 gonococcus clinical strains were used,for jointing and transformiting to colon bacillas and Non PPNG strains respectively.Result 7 PPNG strains were found in 20 gonococus clinical isolates strains,of which carried 4.5 Md plasmid's rate was 40 percent,carried 3.7 Md plasmid's rate was 25 percent;3 PPNG strains with 24.5 Md plasmid could transmit R plasmid to colon bacillas and non PPNG gonorrhoeae strains through joint.2PPNG strains could transmit R plasmid to colon bacillas and non PPNG gonorrhoeae strains through transformation.Conclusion Two types of R plasmids were discovered in Guiyang.PPNG strains could transmitting R plasmids to colon bacillas and non PPNG gonorrhoeae strains through joint and transformation,it's an important reason for PPNG epidmic.