硬脂酸脱氢酶基因ghSAD-1倒位重复构建的棉花遗传转化(英文)

Cotton Transformation Based on Inverted Repeat Construct of stearoyl-ACP△9 desaturase Gene ghSAD-1

以种子特异性表达的大豆凝集素基因启动子、终止子以及棉株体内硬脂酰 -ACP△ 9脱氢酶的基因 gh SAD-1进行倒位重复基因构建 ,以农杆菌介导法转化陆地棉品种 Coker3 1 5,获得了较高的转化频率。PCR、Southern杂交及种子内脂肪酸组分含量分析。结果表明 ,该倒位重复基因的表达及沉默效应在不同的转基因品系间存在差异 ,硬脂酸含量分别由对照的 2 %提高至 4%～3 8.2 % ,而棕榈酸含量则明显降低。高含硬脂酸的转基因品系可作为棉子油品质改良的基础材料 。

A partial inverted repeat based gene construct was produced and cloned into the pBI-Lectin binary vector, using the strong seed specific so ybean Lectin promoter and cotton stearyol-ACP△9 desaturase gene ghSAD-1. The cotton cultivar G.hirsutum cv. Coker315 was then transformed by the co nstruct through Agrobacterium mediated infection. A relatively high tr ansformation freque ncy was obtained. Further analysis by PCR, Southern blotting and fatty acid comp osition analysis demonstrated that the expression or silencing effectiveness of the transgene was not in the same level among various transgenic lines, where th e stearic acid content was increased from 2 percent of the control to a range of 4～38.2 percent. These materials are valuable for the further improvement of cu rrent cottonseed oil in the future.