DNA单链二级结构预测与单链构象多态性分析的一致性研究

The Comparison of Predicted Second Structure of DNA Single Strand with Single Strand Conformation Polymorphism Analysis Results

单链构象多态性 (SSCP)分析是一种简便 ,快速检测DNA突变的方法 ,它在基因突变检测、遗传分析、进化研究等领域有着广泛的应用价值 .但是这种方法的突变检出率随DNA序列不同而变化 ,一般只能达到 70 %～ 80 % .这主要是有的碱基突变对单链DNA的构象影响较小 ,不能通过SSCP检测出来 .将计算机对DNA二级结构的预测结果和实验结果作了对比 ,发现二者有很高的一致性 .这一结果表明计算机的DNA单链二级结构预测分析可用于PCR SSCP分析的辅助设计 。

Single strand conformation polymorphism (SSCP) has been widely used for mutation detecting. But the theory about it is not strong enough to guide application. Recently, many computer programs have been designed for predicting the folding of single strand DNA or RNA. Second structures of three DNA segments with RNAstructure 3.2 have been predicted and have been compared with their results of SSCP. The comparison shows there is closely relation between them. The kinds of prediction can guide operator to select primers for SSCP analysis and accurately estimate mutated ratio.