摘要
分别用活化素 (Activin)、卵泡抑素 (FSP)及其组合 (Activin +FSP)来处理培养的鸭未成熟卵泡颗粒细胞 ,发现在FSH存在与不存在的情况下 ,Activin均能促进FSH受体mRNA的表达 ,且随着Activin浓度的增大 ,其刺激作用增强。FSP自身对FSH受体产生无显著作用 ,但能中和Activin对该受体产生的促进作用。这说明FSP和Activin对颗粒细胞具有自分泌作用 ,二者通过调节FSH受体mRNA的表达而在卵泡的生长发育过程中起着重要作用。
The aim of this study was to investigate the action of activin and follistatin either alone or in combination on FSH receptor mRNA expression. The results showed that activin alone increased FSH receptor mRNA level in the presence or absence of FSH in cultured granulosa cells,and the stimulating effect of activin on FSH receptor level was dose dependent. This effect of activin was inhibited by FSP treatment which alone had no effect on FSH receptor expression. From the results,it can be concluded that activin and follistatin both exert autocrine actions on granulosa cells,and the two factors,possibly by regulating FSH receptor expression,may play important roles in follicular development.
基金
国家自然科学基金 (批准号 3990 0 1 0 6)资助~~
