金雀黄素抑制人肝癌细胞Bel 7402中NF-κB表达的实验研究

Experimental Study of the Inhibiting Effects of Genistein on the Expression of NF-κB in Human Hepatic Cancer Cell Strain Bel 7402

从核转录因子水平探讨金雀黄素抗肿瘤作用机理。采用MTT法测定金雀黄素抑制肿瘤的作用 ;通过免疫组化、WesternBlot及电泳迁移率变动分析 ,分别检测了用药前后 p65和IκBα在人肝癌细胞株Bel 74 0 2中的表达。结果提示金雀黄素有显著抑制肿瘤生长的作用 ,用药后 ,Bel 74 0 2细胞中的 p65表达水平降低 ,而IκBα表达较用药前高。表明金雀黄素的抗肿瘤作用机理与其抑制IκBα降解从而抑制NF -κB功能有关。

The anticancer effects of genistein was studied on the NF-κB level in this experiment. MTT method was used to determine the anticancer effects of genistein, and immunohistochemical method, Western Blot method and electrophoretic migration rate analysis were used to determine the expression of p65 and IκBα in human hepatic cancer cell strain Bel 7402 before and after the genistein administration. The results showed that genistein could markedly inhibit the growth of cancer: and after its administration, p65 expression decreased and IκBα expression increased in human hepatic cancer cell strain Bel 7402. The results implies that the anticancer mechanism of genistein is related with its inhibiting of NF-κB function resulting from its halting IκBα degradation.