三七总皂甙对转化的人胃粘膜上皮细胞增殖的抑制作用

Inhibitory Effects of the Total Saponin of Panax Notoginseng on the Proliferation of the Transformed Human Gastric Mucosal Epithelial Cells

通过四甲基谷氮唑盐实验及软琼脂集落形成试验 ,观察三七总皂甙 (PNS)对永生化的人胚胃粘膜上皮细胞系GES 1以及经过甲基硝基亚硝基胍 (MNNG)转化后的GES 1细胞 (MC细胞 )增殖能力的影响 ,并与维甲酸进行比较。结果表明 ,PNS对GES 1和MC细胞的增殖活性有明显的抑制作用 ,并有随剂量增加作用增强的趋势 ,还能明显抑制MC细胞的软琼脂集落形成能力。维甲酸对细胞的增殖活性和软琼脂集落形成能力也有显著的抑制作用 ,但其产生作用的时间以及作用后细胞形态的变化与PNS作用后有所不同。提示PNS对GES 1及其转化细胞有显著的增殖抑制作用 ,推测其作用机制可能与维甲酸有所不同。

MTT method and the soft agarose colony forming test were used in this study to observe the effects of the total saponin of Panax notoginseng (TSPN) on the proliferation of the immortalized human gastric mucosal epithelial cell line (IHGMECL) GES 1 and MNNG transformed IHGMECL GES 1, in comparison with the effects of retinoic acid. The results showed that TSPN could obviously inhibit the proliferation of both IHGMECL GES 1 and MNNG transformed IHGMECL GES 1 with a tendency towards a dose effect relationship, and exhibit a marked inhibitory effect on the soft agarose colony forming ability of the MNNG transformed IHGMECL GES 1. The results also showed that althouth retinoic acid exhibited similar effects on both the IHGMECL GES 1 and MNNG transformed IHGMECL GES 1, it was different from TSPN in the time of action and the morphological changes induced in the cells. The results imply that the mechanism of the effects of TSPN may be different from that of retinoic acid.