TNF-α对新生大鼠成骨细胞产生一氧化氮的影响

TNF-α INDUCES NITRIC OXIDE PRODUCTION IN NEUBORN RAT CALVARIA OSTEOBLASTIC CELL

目的 探讨肿瘤坏死因子α(TNF -α)对体外培养成骨细胞产生一氧化氮 (NO)的影响。方法 体外培养新生大鼠成骨细胞内加入不同浓度的TNF -α(2 0、30、4 0ng/ml) ,培养 2 4、4 8h ,用亚硝酸盐法测量培养液内NO的变化。结果 在 30 4 0ng/ml浓度的TNF -α作用 4 8h ,NO产生较对照组明显升高 (P <0 0 5 )。 结论 TNF -α可刺激成骨细胞产生NO ,而NO作为一高效细胞毒效应分子与骨丢失有关。

Objective To explore the effects of TNF-α on NO production in Newborn rat calvaria osteoblastic cell.Methods Newborn rat calvaria osteoblastic cells were incubated with TNF-α(20,30,40ng/ml)for 24 and 48H.The nitrite concentrations in the medium was measured by enzyme assay.Results No production was significanly high at 48h at the dose 30.40ng/ml of TNF-α compring to that of control group(P<0.05).Conclusion TNF-α can induce NO produciton.NO may be a potentially factor which promote the bone resorption.