鉴定致病菌毒力因子方法的进展

The progress of method for identifying the virulence factors of virulent bacteria

综述鉴定致病菌毒力因子的分子生物学和遗传分析方法 ,如签名标记法、简并引物 PCR、体内表达技术、差减杂交法、随机引物 PCR、差异显示技术、酵母双杂交系统和噬菌体显示系统等。

This paper discusses some molecular biological and genetic methods for identifying the virulence factors of virulent bacteria, for example, signature tag mutagenesis, degenerate primer PCR, in vivo expression technology, subtractive hybridization, arbitrary primer PCR, yeast two hybrid system and phage display system, et al.