川芎嗪对实验性溃疡性结肠炎治疗作用的研究

Inhibitory Effects of Tetramethylpyrazine on Ulcerative Colitis in Mice

目的：研究川芎嗪对小鼠溃疡性结肠炎的治疗作用及其机制。方法：在小鼠溃疡性结肠炎模型上观察川芎嗪的治疗作用，并用免疫组化ＬＳＡＢ（ｌａｂｅｌｌｅｄ ｓｔｒｅｐｔａｖｉｄｉｎ ｂｉｏｔｉｎ）法检测肠组织中Ｐ选择素的表达，用ＥＬＩＳＡ法检测血浆Ｐ选择素含量。结果：溃疡性结肠炎小鼠结肠粘膜充血、水肿、糜烂或溃疡性形成；粘膜炎症细胞浸润（主要为中性粒细胞）及隐窝脓肿形成；川芎嗪治疗组小鼠未见明显病理变化，疾病活动指数（ＤＡＩ）和病理学评分明显改善。免疫组化和ＥＬＩＳＡ检测发现，溃疡性结肠炎时肠组织Ｐ选择素表达和血中Ｐ选择素水平显著升高，经川芎嗪治疗的小鼠Ｐ选择素明显下降。结论：Ｐ选择素与溃疡性结肠炎密切相关，川芎嗪对溃疡性结肠炎具有治疗作用。

Background/Aims: To investigate the inhibitory effects and mechanisms of tetramethylpyrazine (TMP) on ulcerative colitis in mice. Methods: The mice were randomized into three groups. A normal control group (n = 10) received tap water orally for 7 days. Colitis was induced by feeding mice with 5% DSS for 7 days, and then either TMP (8 mg/100 g body weight, n = 11) or normal saline solution (n= 9) was injected intraperitoneally in mice daily from day 1 to day 5. On day 7 the mice were killed and co- litis was evaluated histologically and clinically. Results f Treatment with TMP significantly attenuated colonic damage, neutrophil infiltration and disease activity index (DAI). P-selectin expression was found positive in saline-treated group. Meanwhile, the plasma level of P-selectin was higher in saline- treated group than in TMP-treated group. Conclusions f The results suggest that P-selectin plays an important role on ulcerative colitis, and TMP inhibits the P-selectin expression and inflammation in mice with ulcerative colitis.