双稠哌啶类生物碱对5个环境细菌菌株的抑制作用

Bacteriostatic Effects of Quinoliziding Alkaloids on Five Environmental Bacterial Strains

测定了槐定碱、槐胺碱、苦参碱、野靛碱、氧化苦参碱和苦豆草总生物碱对大肠杆菌、产气杆菌、变形杆菌、枯草杆菌、白色葡萄球菌 (即表皮葡萄球菌 )的最低抑菌浓度 (MIC)。结果表明 5种生物碱和苦豆草总生物碱对 5个环境细菌菌株的生长均产生了明显的抑制作用。5种生物碱对 5种环境细菌的最低抑菌浓度除了野靛碱对变形杆菌为 5× 1 0 - 3 mol/L ,其它均在 2× 1 0 - 2 ～ 5× 1 0 - 2 mol/L之间。然而苦豆草总生物碱对 5种环境细菌的最低抑菌浓度却明显较 5种生物碱的为低 ,其中对大肠杆菌和枯草杆菌的最低抑菌浓度达到 5× 1 0 - 3 mol/L。结合双稠哌啶类生物碱在植物中的含量和分布 ,讨论了双稠哌啶类生物碱在开发防治农林细菌病害及医药产品方面的应用价值和在植物化学生态学中的防御作用。

The minimum inhibitory concentration(MIC)of sophoridine ,sophoramine,matrine,cytosine,oxymatrine and the total alkaloids of Sophora alopecuroides L.on bacterial strains,Escherichia coli,Enterobacter aerogenes ,Proteus vulgaris,Bacillus subtilis,Staphylococcus epidermidis was tested.The results shown that the five alkaloids and the total alkaloids of S.alopecuroid es significantly inhibited grouth of the five environmental bacterial strains. MIC of the five alkaloids on the five environmental bacterial strains were betwe en 2×10 -2 mol/L and 5×10 -2 mol/L,except 5×10 -3 mol/L for cytosine on P.vulgaris,.However,MIC of the total alkaloids of S.alopecuro ides on the five environmental bacterial strains were significantly lower than that of the five alkaloids,in which MIC on E.coli and B.subtilis reache d 5×10 -3 mol/L.Value of developing alkaloid products applied in controll ing agricultural and forest bacterial disease and medicine,defensive effects of quinolizidine alkaloids in chemical ecology of plants were discussed in connecti on with concentration and distribution of quinolizidine alkaloids in plants.