渗透胁迫下芦苇胚性愈伤组织中脯氨酸含量的变化及外源ABA的影响

Changes of Free Proline Contents of the Calluses from Reed Ecotypes to the Osmotic Stress and Exogenous ABA

研究了 PEG- 6 0 0 0胁迫下两种生态型芦苇胚性愈伤组织游离脯氨酸含量的变化及外源 ABA对其变化的影响。结果表明 :在 10 %～ 30 % PEG的胁迫下脯氨酸迅速积累 ,沙生芦苇胚性愈伤组织 (SREC)的积累量明显高于水生芦苇胚性愈伤组织 (WREC)。施加 10μmol/ L ABA后 WREC的脯氨酸含量大幅度升高 ,但对 SREC的影响较小。

The changes of free proline contents of the calluses from two kinds of reed ecotypes have been detected. The results were as below; There was a rapid proline accumulation in both of the embryogenic calluses of sand reed (SREC) and water reed (WREC) to the PEG osmotic stress treatment from 10% to 30%, and the proline content of SREC was much higher than that of SREC. It also showed that response of WREC to exogenous ABA (10μmol/L) was much higher than of SREC in the aspect of prolineaccumulation.