小鼠腹腔巨噬细胞对红系祖细胞增殖的影响

STUDY OF THE REGULATION OF ERYTHROPOIESS BY MOUSE PERITONEAL MACROPHAGES

应用造血祖细胞体外培养技术,观察了小鼠腹腔巨噬细胞对早期红系祖细胞(BFU-E)及晚期红系祖细胞(CFU-E)增殖的影响。结果表明,小鼠腹腔贴壁巨噬细胞对CFU-E表现为刺激作用;对BFU-E增殖依培养体系条件不同而表现为刺激和抑制双重作用。巨噬细胞培养上清液也表现相似的作用。提示巨噬细胞具有促红细胞生成素(EPO)和爆式集落促进活性(BPA)样活性。

By using the techniques of hemopoietic progonitor culture, we studied the effect of mouse peritoneal macrophages on the proliferation of the erythroid burst forming units (BFU-E) and the erythroid colony-forming units (CFU-E). The results showed that macrophages stimulated the CFU-E significantly, and that the growth of BFU-E was enhanced in the absence of additional BPA, but was inhibited when BPA was added in the culture system. The supernants of cultured peritoneal macrophages presented similar effects.The mechanism of the regulation of erythropoiesis by mouse peritoneal macr-ophages is discussed. Our observations suggest that macrophages may stimulate EPO-like and BPA-like activity.