产生硫酸软骨素酶厌氧菌导致大骨节病的实验研究

AN EXPERIMENTAL STUDY OF CHONDROITIN SULFATASE-PRODUCINGANAEROBIC BACTERIA-AN ETIOLOGICAL STUDY OF KASHIN-BECK DISEASES

为证实产生硫酸软骨素酶的厌氧菌是引致大骨节病的主要因素进行了实验研究。从山东省益都(青州市)和陕西省永寿病区分离出产酶菌。将该菌制成菌粮饲养小白鼠30d后,有84.61%(11/13)的小白鼠关节软骨组织出现类似人类大骨节病的病理学改变。对大骨节病的4个特点,3种病因假说以及改水、改善卫生可以降低发病率等作了讨论。提出产生硫酸软骨素酶厌氧菌引致大骨节病的新假说。

Based on the analysis of a large number of facts collected by the authors and other workers referring to the intimate relationship between water supply and pathoge- nesis of Kashin-Beck disease in Yidu area, the authors carried out an examination of chondroitin sulfatase-producing anaerobic bacteria in the drinking water in Yidu and Yongshon areas, and observed the pathogenical changes in the articular cartilage of the experimental mice fed on food mixed with the cultures of these anaerobia. Three species-bacteroides, propionbacterium and petococcus-were isolated from the water of the endemic areas. And 84% of the experimental mice fed on food mixed with anae-robic cultures developed necrosis and degeneration of the articular cartilage after 30 days. The pathological changes were similar to those of the human KBD in its early stage. As the feeding period was prolonged, the pathological damage in the joint became more serious. The above suggests a new concept of the etiology of this disease-the chondroitin sulfatase-producing anaerobia in the water contaminated with human or do- mestic animal feces play the major role in inducing this disease.