人参总皂甙诱导人内皮细胞表达造血生长因子的实验研究

Experimental study on expression of hematopoietic growth factors from human endothelial cell induced by TSPG

目的：研究和论证人参促进血细胞发生及“补气生血”的现代生物学机理。方法：采用造血生长因子生物活性检测与免疫细胞化学等技术，研究经人参总皂甙（ＴＳＰＧ）诱导的人脉静脉内皮细胞株（Ｅｃｖ３０４）上清液（ＨＥｃＣＭ）中造血生长因子的活性及ＧＭ－ＣＳＦ、ＩＬ－３和ＩＬ－６在内皮细胞的表达。结果：ＴＳＰＧ体外诱导制备的ＨＥｃＣＭ能显著促进ＣＦＵ－ＧＭ和ＣＦＵ－Ｍｉｘ的增殖；同时，ＴＳＰＧ能不同程度地促进内皮细胞表达ＧＭ－ＣＳＦ、ＩＬ－３和ＩＬ－６蛋白。结论：本研究提示ＴＳＰＧ能上调造血微环境中的内皮细胞分泌造血生长因子来调节血细胞的生长，这或许是人参“补气生血”的现代生物学机理之一。

Objective：To study modern biological mechanism for hematonic of Ginseng.Methods：By using the techniques of biological assay of hematopoietic growth factor and immunocytochemistry, the activity of hematopoietic growth factors of the conditioned culture media of human umbilical vein endothelial cell (Ecv304) (HEcCM) and the expression of GM-CSF,IL-3,IL-6 in the endothelial cell induced by Total Saponins of Panax Ginseng (TSPG) were studied .Results：HEcCM prepared with TSPG markedly promoted the proliferation and differentiation of CFU-GM and CFU-Mix. The expression of GM-CSF,IL-3 and IL-6 in the endothelial cell induced by TSPG was much stronger than that of the control.Conclusion ：We speculate that TSPG augmented endothelial cell capacity to support hematopoiesis,and this property might result from the upregulation of hematopoietic growth factors expression.