牛磺酸对细胞因子诱导的胰岛细胞凋亡的影响

The influence of taurine on IL-1β,TNF and IFN-γ induced apoptosis of rat pancreatic islet cells

目的：观察牛磺酸对白细胞介素－１β（１Ｌ－１β）、肿瘤坏死因子（ＴＮＦ）和γ－干扰素（ＩＦＮ－γ）诱导的胰岛细胞凋亡的影响。方法：应用体外单层培养的Ｗｉｓｔａｒ大鼠胰岛细胞，分别检测ＩＬ－１β、ＴＮＦ和ＩＦＮ－γ以及牛磺酸对胰岛细胞凋亡细胞百分率，培养液中ＮＯ含量及ＮＯＳ活性以及ＤＮＡ片段的影响。结果：ＩＬ－Ｉβ、ＴＮＦ和ＩＦＮ－γ联合可诱导胰岛细胞凋亡率及ＤＮＡ片段明显增加，同时ＮＯ含量和ＮＯＳ活性亦明显升高（Ｐ＜０．０１）；牛磺酸能阻断上述细胞因子的作用（Ｐ＜０．０１），且有剂量依赖性。结论：ＮＯ可能是介导上述细胞因子引起胰岛细胞凋亡的重要途径之一。牛磺酸能够改善细胞因子诱导的胰岛细胞凋亡，其机制可能与抑制ＮＯＳ活性从而减少ＮＯ的生成有关。

Objective： To observe the role of taurine on apoptosis of isolated pancreatic islet cells induced by combination of IL-1β,TNF and IFN-γ.Methods： Isolated pancreatic islet cells from Wistar rat were incubated in monolayer in vitro.NO production,NOS activity ,apoptotic percentage of islet cells and DNA fragmentation in pancreatic islet cells incubated with combination of IL-1β,TNF and IFN-γ， taurine were measured .Results:Combination of IL-1β,TNF and IFN-γ could induce a significant increase in apoptotic percentage of pancreatic islet cells and DNA fragmentation.NO production and NOS activity also markedly increased (P＜0.01)，which were blocked by addition of taurine to the pancreatic islet cells (P＜0.01)。Conclusion:NO might be one of the important ways of inducing apoptotic cell death of pancreatic islet cells by a combination of IL-1β,TNF and IFN-γ.Taurine attenuates the cytokines-induced β cells apoptosis， the mechanism of which may be the inhibition of NOS and the decreasing of NO production.