Fmc,镰刀菌来源的多巴胺1受体抑制剂

Fmc, a receptor inhibitor of dopamine 1 receptor from Fusarium

应用以中枢神经系统为主的八个体外受体筛选系统 ,从 15株真菌和 12株放线菌中筛选出能产生具有与多巴胺 1受体竞争性结合活性的镰刀菌 Fusariun moniliflore;从其菌体中分离出活性成分 Fmc。通过元素分析和质谱 ,确定其分子式为 C1 0 H1 3O2 N。综合紫外光谱、红外光谱、1 H- NMR和 1 3C- NMR等图谱数据的解析 ,确定 Fmc的结构与镰刀属真菌 (Fusarium moniliflore)发酵液分离出来的夫西地酸 (fusaric acid)一致 ,化学命名为 :5 -正丁基吡啶酸。但关于其作用于中枢神经系统多巴胺

Using a method for screening of central nervous system receptor antagonists from microorganisms in receptor binding assay in vitro , a positive strain named Fusarium moniliflore was obtained from 15 fungi and 12 actinomycetes whose metabolites exhibited inhibitory activities on dopamine 1 receptor. Bioactive compound--Fmc was isolated from its mycelium. By elemental analysis and MS data, its formula was determined to be C 10 H 13 O 2N; based on the UV, IR, 1H NMR, 13 C NMR etc., spectral data and physico chemical properties of Fmc, the structure of Fmc (5 butylpicolinic acid) was elucidated and was identical with that of fusaric acid .