内蒙籍汉族儿童过敏性紫癜关节胃肠道肾脏受累与HLA-DQA1的相关性

Correlation between HLA-DQA1 allele and involved joints,gastrointestine and kidney in anaphylactoid purpura Han juvenile inhabited in lnner Mongolia

目的 探讨内蒙籍汉族儿童过敏性紫癜 (AP)关节、胃肠道、肾脏受累与HLA DQA1等位基因的相关性。方法 采用聚合酶链反应 序列特异性引物 (PCR SSP)技术 ,在内蒙籍汉族无血缘关系及其他风湿性疾病史或家族史的人群中 ,收集儿童AP累及关节 45例、胃肠道 39例和肾脏 32例分别与 90名健康儿童HLA DQA1等位基因作型别分析。结果 AP累及关节、胃肠道和肾脏DQA1 0 30 1等位基因频率分别为 2 8 5 %、2 4 9%和 2 5 % ,均明显高于对照组 10 6 % ,差异有非常显著性 (χ2 分别为 12 0 0 8、7 6 39和 6 882 ,P值分别为 0 0 0 1、0 0 0 6和 0 0 0 9,均 <0 0 1,RR分别为3 83、3 0 9和 3 11,EF分别为 0 36、0 2 95和 0 2 97) ;而DQA1 0 30 2等位基因频率分别为 5 7%、3 9%和 6 5 % ,明显低于对照组 19% ,差异有非常显著性 (χ2 分别为 8 35 2、10 6 33和 5 5 5 7,P值分别为 0 0 0 4、0 0 0 2和 0 0 18,前二者P <0 0 1,后者P <0 0 5 ,RR分别为 0 2 4、0 15 9和 0 2 72 ,PF分别为 1 0 9、1 80和 0 92 )。结论 HLA DQA1 0 30 1等位基因可能是内蒙籍汉族儿童AP累及关节、胃肠道和肾脏发病单体型中一个遗传易感基因 ,而DQA1 0 30

Objective To analyse the genetic susceptibility of HLA DQA1 allele to anaphylactoid purpura with involved joints (APIJ),gastrointestestine (APIG) and kidney (APIK) in juvenile Han inhabited in Inner Mongolia.Method There were 45 children with APIJ,39 with APIG and 32 with APIK and 90 normal children controls,all of which were proved to be no sibship and no rheumatic diseases and family history.Of them,HLA DQA1 allelic types were analysed by polymerase chain reaction sequence specific primer (PCR SSP) technique.Results Gene frequencies of HLA DQA10301 in APIJ,APIG and APIK groups (28 5%,24 9% and 25%,respectively) were significantly higher than those of control group (10 6%),and the difference had statistical significance( χ 2=12 008,7 639 and 6 882; P =0 001,0 006 and 0 009;RR=3 83,3 09 and 3 11;EF=0 36,0 295 and 0 297,respectively).In contrast,HLA DQA10302 allelic frequencies in APIJ,APIG and APIK groups (5 7%?3 9% and 6 5%,respectively) were significantly lower than those in control grorp (19%) respectively,and the difference had statistical significance ( χ 2=8 352,10 633 and 5 557; P =0 004,0 002 and 0 018;RR=0 24?0 159 and 0 272;PF=1 09?1 80 and 0 92,respectively).Conclusion The allele of HLA DQA10301 may be a susceptible gene for the Han children with APIJ,APIG and APIK in Inner Mongolia,while HLA DQA10302 gene may be the protective one.