肝硬化时肝细胞胰岛素受体和酪氨酸蛋白激酶表达的定量研究

Quantitative analysis of the expression of insulin receptor and tyrosine protein kinase of patients with hepatic cirrhosis

目的 探索肝硬化时肝组织细胞胰岛素受体（IR）和酪氨酸蛋白激酶（TPK）含量的变化规律。 方法 应用图象分析系统对12例血清HBV标志物阳性的肝炎后肝硬化患者肝组织石腊包埋切片对抗胰岛素受体和酪氨酸蛋白激酶抗体免疫组化标记物的定量测定。 结果 肝硬化糖耐量试验（GTT）异常组和GTT正常组IR含量分别为（41.44±6.47）AU和(43.36±4.78）AU，较对照组［(48.11±4.42）AU］显著减少（P＜0.01），肝硬化GTT异常组和GTT正常组IR含量无显著性差异（P＞0.05）；TPK表达量：肝硬化GTT异常组较GTT正常组显著性减少，分别为(41.31±5.55）AU和（51.16±5.83）AU。 结论 肝硬化时肝细胞IR减少；肝硬化时GTT异常与肝细胞胰岛素受体酪氨酸蛋白激酶活性减退有关。

Objective To investigate the relationship between the expression of insulin receptor (IR) and the content of tyrosine protein kinase (TPK) in patients with hepatic cirrhosis. Methods Glucose tolerance test (GTT), insulin, c-peptide, insulin antibodies in serum, the expression of IR and TPK were quantitively analysed in patients with hepatic cirrhosis. The amount of IR and TPK was evaluated by immunohistochemical quantitative analysis using image analyzer in 12 patients with positive HBV marks and cirrhosis. Insulin and c-peptide in serum were determined by immunoenzymemetric assay (IEMA) and insulin antibodies in serum determined by RIA. Results IR amount in normal and abnormal GTT patients with hepatic cirrhosis was signifficantly less than that in control group (P<0.01). TPK amount in liver of patitents with abnormal GTT was less than that of normal GTT (P<0.01). Conclusions IR decreases in cirrhotic patients. Abnormal GTT has relation to reducing TPK activity. [