人工角膜支架材料在动物体内应用的生物学反应

The biological response of synthetic materials implanted in animal

目的 探讨 3种人工角膜支架材料经氩等离子体 (Argonplasma)处理后植入兔角膜对角膜组织的生物学反应的影响。方法 选用 3种支架材料 :由 80 %的聚丙烯和 2 0 %聚丁烯化合而成的多孔材料 ;Polyester多孔材料 ;Ex pendedpolytetrafluoroethylene。处理组材料经Argonplasma处理。将盘状支架材料植入兔角膜囊袋内。 结果 材料经处理后角膜水肿较未处理明显减轻 ,8周后 3种材料中角膜水肿均消失。材料 1,2植入时新生血管出现延迟 ,范围小 ,炎细胞浸润明显减少。术后 6周纤维细胞迁徙入材料内。当材料植入角膜后角化蛋白 (KS)含量降低 ,Dermatan(DS)含量增高 ,并有大分子葡萄糖聚糖 (GAG)出现。结论 Argon plasma处理可明显减轻兔角膜炎症反应 ,增加材料的组织相容性。

ObjectiveTo compare the biological response of the corneal stroma with three porous materials treated with argon plasma.MethodsThree porous materials were chosen:a melt blown microfiber web of polybutylene:polyproplene(80∶20),polyester spun laced fabric,and an expended polytetrafluoroethylene.Fifty percent of each material was modified using argon plasma treatment.Discs with 6mm diameter were inserted into interlamellar stromal pockets and followed for a period of 12 weeks.Clinical examination was performed weekly.ResultsPretreatment of the discs reduced corneal edema and neovascularization.The presence of the collagen in the disc indicated that cells had migrated into the disc and deposited an extracellular matrix protein.ConclusionArgon Plasma treatment increases the biocompatibility of all materials.Fibroplasia occurred most rapidly into the polyester discs.The distribution of collagen was not altered by the materials.