睑结膜部炎症者肺炎衣原体、沙眼衣原体核酸检测

The nucleic acid of Cpn and Ct in patients suffering palpebral conjunctivitis

目的 探讨我国睑结膜部炎症者病灶部是否存在肺炎衣原体 (Cpn)。 方法 收集 39例慢性结膜炎、沙眼患者的睑结膜病灶部刮取物 ,应用nestedPCR(nPCR)技术检测肺炎衣原体 (Cpn)和沙眼衣原体 (Ct)核酸。并随机抽取 2例CpnnPCR阳性产物进行全自动DNA测序 ,加以佐证。 结果 39例患者中Cpn阳性 13例 ( 33 3% ) ,Ct阳性 5例( 12 8% )。慢性结膜炎和沙眼病例均有Cpn和Ct阳性者。 2例CpnnPCR阳性产物经全自动DNA测序与Cpn(CWL—2 9株 )高度同源。结论 我国睑结膜部炎症者病灶部存在Cpn。

ObjectiveTo discuss the existence of Cpn palpebral conjunctivitis.MethodsThe scrapings from the palpebral conjunctivitis of 39 patients suffering from chronic conjunctivitis and trachoma were collected and the nucleic acid of Cpn and Ct by means of nested PCR (nPCR) was investigated and then the positive products of Cpn and nPCR were selected randomly to make the automatic DNA test.ResultsThere were 13 cases of the positive of Cpn (33 3%) and 5 cases of the positive of Ct (12 8%) among the 39 cases.The positive of Cpn and Ct existed in the cases of male and female patients.The test of the positive products of Cpn nPCR in 2 cases by the automatic DNA test showed the same source.ConclusionCpn exists in the focus of the inflammation of conjunctivitis.