BMP-2在成纤维细胞对BMP-3表达的调节作用

The Effect of BMP-2 on the Expression of BMP-3 of Fibroblast

目的 :观察在BMP -2作用下 ,成纤维细胞表达BMP -3的情况 ,探讨BMP -2促进成纤维细胞成骨表型表达的机制。方法 :向培养的成纤维细胞内添加BMP -2 ,当细胞生长到 6 0 %时免疫组化观察正常与BMP -2作用下的成纤维细胞内表达BMP -3的情况。结果 :正常成纤维细胞内无BMP -3表达 ,在BMP -2作用下成纤维细胞出现BMP -3表达。结论 :在成纤维细胞 ,BMP -2可促进BMP

Objective: To observe the expression of BMP-3 of fibroblast,for better understanding the mechanism of expression of osteogenic phenotype of fibroblast under the effect of BMP-2.Method:Add BMP-2 to the fibroblast,histoimmunochemistry to observe the expression of BMP-3 when the cell was 60% of the culture plate.Take the normal fibroblast as the control.Result:There is no BMP-3 expressed in the normal fibroblast.BMP-3 could be detected under the effect of BMP-2.Conclusion:BMP-2 could stimulate the expression of BMP-3 in the fibroblast.