一次性高糖发酵L-缬氨酸菌种的选育及发酵条件

Selective breeding process and fermenting condition of L-valine in highglucoside

以产L 缬氨酸Apv 5为出发菌株 ,经过硫酸二乙酯诱变处理 ,获得了一株在高糖培养基上生长良好的菌株Apv 6 ,在 30t发酵罐上进行工业化大生产。一次性投糖 18%～ 2 1% ,6 0h左右发酵结束。

Using L valine as producing strain and metagenizing with diethyl sulfate, we obtain well grown strain Apv 6, after 60 hour's one kick fermentation in 18%～21% high glucoside, accomplished in industrialized mass production with a 30 ton tank. This paper reports the breeding process and fermentation condition.