摘要
采用菠萝蛋白酶和木瓜蛋白酶制剂对从珍珠贝壳珍珠层中提取的珍珠壳角蛋白进行酶促降解实验,用紫外分光光度法检测降解产物小肽及酪氨酸在275nm波长的紫外吸收值,以观察分析这两种植物蛋白酶对壳角蛋白的降解效果,探讨其酶促降解的条件实验。结果表明:两种植物蛋白酶对壳角蛋白都有一定的降解作用,菠萝蛋白酶降解效果优于木瓜蛋白酶,它在底物浓度小于0.2%时,与酶解产物呈线性关系,酶浓度一般宜保持在100μg/ml以下,酶促反应时间以10h为佳。
Enzymolysis test about the conchiolin extracted from the shell nacre of pearls by using pineapple protease and papain was made to examine the UV absorbance of peptide and tyrosine at the wavelength of 275 nm with ultraviolet spectrometry.After observing and analyzing conchiolin enzymolysis effect of the two kinds of plant protease,we probed into the conditions of its enzymolysis.The conclusion showed.The two kinds of plant protease have had particular enzymolysis effects and the enzymolysis effect of pineapple protease was higher than the papain's.It appeared a line relation ship with enzymolysis products when the substrate concentration was less than 0.2%.It was optimal when the enzyme concentration was kept under 100μg/ml and enzymolysis time about 10 hours.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2001年第12期19-22,共4页
Food Science
基金
江苏省教委自然科学基金资助项目(JW970062)
