核桃仁活性成分的提取及体外抗氧化活性研究

Study on Extraction and Antioxidative Activity of the Activated component of Juglans regial

用95%乙醇、乙酸乙酯、正乙烷依次萃取核桃仁的可溶成分。用化学发光法、DPPH法、TBA法测定各提取物的抗氧化活性。结果表明:核桃仁各取物对DPPH自由基均有清除作用,以95%乙醇提取物最佳,乙酸乙酯提取物次之;95%乙醇提取物对亚油酸自氧化体系的抑制作用也强于乙酸乙酯提取物,而正己烷提取物则表现出促氧化作用,在以Fe2+及Fenton反应催化的亚油酸脂质过氧化体系中,95%乙醇提取物的抑制作用强于同浓度的茶多酚;此外,95%乙醇提取物对碱性连苯三酚体系产生的超氧阴离子自由基(O2-.)有较强的清除作用,但对VC-Cu2+-酵母-H2O2体系产生的羟基自由基(·OH)清除作用较差。

Soluble components of Juglans regial were extracted uging 95% ethanol,ethylacetate,n-hexane one by one and their antioxiative activies were detrmined using Chemiluminescence,DPPH and TBA method.The results showed that all kinds of extracts of Juglans regial have had scavenging effects on DPPH radical.The 95% ethanol extracts showed the highest activities,where as ethylacetate extracts the second.The 95% ethanol extracts gave stronger resistance to the autoxidative system of linoleate than ethylacetate ones.On the contrary the n-hexane extracts promoted the oxidation.In the linoleate per oxidative system catalyzed by Fe2+ and Fenton reaction,the inhibiting action of 95% ethanol extracts was stronger than tea polyphenols with the same concentration.Besides,95% ethanol extracts showed strong scavenging effects on superoxidized anionic radical(O2·) Produced by system of alkaline Bipheng Triphenol,but give weak scavenging effects on hydroxy1 radical(·OH) Produced by VC-Cu-barm-H2O2 system.