摘要
采用高效液相色谱法对影响银杏叶提取物中黄酮类化合物水解效果的不同因素进行了研究,对样品的回收率进行了评价,并对四种定量计算方法进行了比较。通过正交试验的极差和方差分析对水解过程的盐酸浓度、水解时间以及甲醇水的体积比进行了优化:样品溶解于1.0mol/L盐酸甲醇溶液,80℃水解1h,供进行HPLC分析。结果表明酸浓度和甲醇溶液浓度对水解效率的影响显著,而水解时间对其影响不显著;对照品及换算因子的选用不同对定量结果的影响较小。
High-performance liquid chromatography (HPLC) was used to evaluate the influence of different variables affecting the hydrolysis of flavonoids in extract of Ginkgo biloba L.(EGb).The hydrolysis recovery was estimated and four kinds of quantiative methods were also been compared.The effects of concentration of HCl in methanol,time and methanol/H2O volume ratio can be interpreed by observing the variation of quantitative results.The acid-hydrolysis conditions optimized by using the orthogonal design method and the anlysis of ranges and variances were obtained as follows:sample soluted in 1.0mol/L HCl-methanol,hydrolyzed at 80℃ for 1.0h,then analyzed by HPLC.Results show that the concentration of HCl and methanol affected the hydrolysis efficiency more markedly than time,difference standard solutions and convert factor cause less imparity to quantiative results.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2001年第12期66-68,共3页
Food Science
基金
江苏省"九五"重大工业科技攻关项目(BG98020-2)
