摘要
报道了用分光光度法测定大黄中大黄素含量的方法 .实验发现 ,微量的大黄素与 Na OH呈显色反应 ,当 p H大于 12 .0 0时 ,在 5 30 nm处有最大吸收峰 ,且大黄素在 1～ 2 0 0 μg范围内与吸光度呈良好的线性关系 ,回归方程为 :Y=0 .0 2 9X+ 0 .0 0 2 ,r=0 .9994 ,检出限为 0 .5 μg.测定了在Tween80 - (NH4) 2 SO4液固萃取体系萃取大黄中大黄素的含量 。
The determination of rheum emodin in rheum by spectrophotometry is reported in this paper.Trace rheum emodin has color reaction in the NaOH medium.The results show an absorption maximum at 530nm . When pH>12.0, the linear range of determination is 1～200 μg/mL. Regression equation: Y=0.029X+0.002, r =0.9994 ,and the determination limit is 0.5μg. When the trace rheum emodin is extracted from rheum by using Tween80 (NH 4) 2SO 4liquid solid extraction system, this method is satisfactorily applied to determinate the content of rheum emodin . The present method is sensitive, rapid, simple, and easy to popularize.
出处
《中南民族学院学报（自然科学版）》
2001年第4期13-16,共4页
Journal of South-Central University for Nationalities(Natural Sciences)
关键词
大黄素
分光光度法
液固萃取
测定
天然药物
有效成分
rheum emodin
spectrophotometry
thin layer chromatography
liquid solid extraction
