乳腺癌术后胸膜转移性胸水TIL的细胞毒活性研究

A study on TILs of the pleural effusion cytotoxcity with metastasis after the breast cancer operation

目的:探讨乳腺癌术后胸膜转移引起的胸水中肿瘤浸润淋巴细胞(ＴＩＬ)毒活性。方法:采用生物技术,提纯乳腺癌胸膜转移所致胸水中的ＴＩＬ和相应的肿瘤细胞,经过一定时间诱导培养后,在７天、１４天、２１天和２８天分别检测ＴＩＬ对自身肿瘤细胞和Ｋ５６２细胞的杀伤活性。结果;该ＴＩＬ对自身肿瘤细胞和Ｋ５６２细胞的杀伤活性无显著性差异,２１天时细胞毒活性最强。结论:ＴＩＬ诱导培养２１天时具有较强的生物活性,此时进行临床应用可能取得最佳疗效。

ve:To investigate the cytotoxcity of tumor infilating lymphcoytes(TIL) on the metastastic pleural effusion after the operation of breast cancer. Methods: TIL and breast cancer cells in pleural effusion cells were purified through biotechnology and the cytotoxcity of TIL on the tumor cells and K562 cells on 7th、 14th、 21th and 28th days after cell culture were tested respectively. Results: There were no significant difference between the cytotoxcity of TIL on the tumor cells and that on the K562 cells statistically. The highest cytotoxcity was found on the 21th day. Conclusions : The biology activity of the TIL was the highest when they were cultured until the 21th days. Therefore, they will be most effective on malignant pleural effusion caused by matastastic breast cancer clinically.