卵巢切除后大鼠骨小梁形态的实验研究

An experimental study of trabecular bone histomorphology in ovariectomized rats

本实验目的是建立大鼠卵巢切除后骨质疏松模型 ,分析骨质疏松后骨小梁节点数减少而游离末端数增加的原因。选用 3月龄雌性Wistar大鼠 ,分OVX和sham组。取大鼠右胫骨干骺端 ,对骨小梁微构筑进行观察。结论为OVX后骨重建活跃 ,St、Nd St区最显著 ,OVX后MAR先升高后降低 。

Objective Using ovariectomized rats as osteoporosis models to explore the cause of decrease in the number of trabecular nodes and increase in the number of free ends. Methods Thirty six 3 month old female Wistar rats were divided into ovariectomized (OVX) and sham operated(sham) groups equally. The surface of trabecular microarchitecture in proximal tibia metapheyses was observed microscopically. Results 1)Trabecular bone volume decreased significantly in OVX group compared with sham group at 12 weeks ( P <0 05).2)Mineral appositional rate (MAR) in OVX group was slightly higher than that of in sham group at 4 seeks( P <0 05). From 8～12 weeks, the MAR in OVX group was much lower than in sham group( P <0 01). 3)From 4 weeks to 12 weeks, the activity in the St and Nd St regions on the surface of trabecular microarchitecture was significantly increased,compared with other regions in either OVX group or sham group. Conclusion Bone remodeling is active in ovariectomized rats. The activity in the region St and Nd St on surface of trabecular microarchitecture is significantly increased,compared with other regions after OVX. The MAR increases for a short time and then decreases. This might be the cause of the decrease in the number of trabecular nodes and increase in the number of free ends.