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凋亡相关基因在急性肝损害中表达的变化 被引量:1

The change of apoptosis-related genes expression in the acute liver injury
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摘要 目的探讨凋亡相关基因表达对急性肝损害的影响。方法用脂多糖(LPS)造成大鼠急性肝损伤的模型 ;用Tunel染色法检测肝细胞凋亡 ,用免疫组化法测定bcl_2、bax、Fas、Fasl凋亡相关基因在肝内的表达。结果Tunel法检测出细胞凋亡高峰时间在LPS处理后15~18h。免疫组化法检测出bcl_2、bax、Fas、Fasl凋亡相关基因在肝内表达的时相性变化。 Objective To investigate the effect of AP_related gene′s expression on the acute liver injury.Methods The model of acute liver injury was formed by i.p.LPS.Liver cells of AP were measured by means of Tunel.The expression of AP_related genes bcl_2?bax?Fas?Fasl was measured by means of histochemistry immunoassay in liver.Results The number of AP cells peaked at 15~18h.The change of expression of AP_related genes was measured in liver at different time by means of histochemistry immunoassay.Conclusions The AP_related genes triggered and accelerated the AP in liver cells.
出处 《中国航天医药杂志》 2001年第6期14-16,共3页 Medical Journal of CASE
关键词 凋亡相关基因 急性肝损害 免疫组化法 Apoptosis_related gene Acute liver injury
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