牙周炎患者龈沟液白细胞介素-1β检测及意义

Detection and Clinical Benefits of Interleukin-1β in Gingival Crevicular Fluid in the patients with Periodontitis

目的 :检测牙周炎患者龈沟液 (gingival crevicular fluid,GCF)中白细胞介素 - 1β(interleukin- 1β,IL - 1β)的含量变化 ,探讨 IL - 1β在牙周炎发病中的作用。方法 :用滤纸条浸润法采集正常成年人及成年人牙周炎患者治疗前后 GCF样本 ,用酶联免疫吸附法 (EL ISA)检测 GCF中 IL- 1β含量变化。结果 :牙周炎患者龈沟液中 IL- 1β水平显著高于正常对照组 (P<0 .0 1) ,牙周炎治疗组患者龈沟液中 IL- 1β水平较牙周炎组显著降低 (P<0 .0 1)。龈沟液中 IL- 1β水平分别与牙龈指数、探诊出血指数、牙周袋深呈显著正相关。结论 :IL - 1β参与牙周炎的发生和发展 ,GCF中 IL - 1β可作为检查牙周病活动情况的一项较为敏感的指标。

Objective:To detect the interleukin 1β (IL 1β) level in gingival crevicular fluid (GCF) in the patients with periodontitis so as to evaluate the role of IL 1β in periodontitis.Methods:IL 1β levels in GCF were detected by ELISA following careful sampling of GCF with the standardized filter strips.Results:IL 1β levels in GCF of patients were significantly increased as compared to those of the control group ( P <0.01),the therapeutic group showed lower IL 1β levels than those of the periodontitis group ( P <0.01).The positive correlation were found between IL 1β and gingival index,bleeding index and probing depth.Conclusion:IL 1β might play an important role in the occurence and development of periodontitis. IL 1β level in GCF may be a sensitive marker for periodontal tissue inflammation.