摘要
用BamHⅠ和XbaⅠ两种限制酶酶切质粒 pKG2 7,获得了编码F2 6G(F2 6G :furostanolgly coside 2 6 O β glucosidase)的cDNA ,然后将其重组到表达载体 pET 2 2b上而得到pET 2 2b CSF2 6G ,利用大肠杆菌E .coliBL2 1进行表达 ,用胞内可溶蛋白进行酶反应 ,通过TLC、HPLC分析证明重组菌表达出高活性的F2 6G酶 。
The full length cDNA of furostanol glycoside 26 O β glucosidase was obtained by the digestion of pKG27 at the sites of BamH Ⅰ and Xba Ⅰ.It was ligated to the pET 22b vector,and expressed in E.coli BL21 strain.It was achieved that the bioconversion of furostanol glycoside to spirostanol glycoside by the cell lysate from E.coli BL21/pET22b F26G.
出处
《中国药物化学杂志》
CAS
CSCD
2001年第6期326-328,共3页
Chinese Journal of Medicinal Chemistry
关键词
F26G
重组
表达
呋甾皂苷
螺甾皂苷
生物转化
F26G
recombination
expression
furostanol glycoside
spirostanol glycoside
bioconversion
