血管内皮生长因子在妊高征胎盘中的表达研究

STUDY ON THE EXPRESSION OF VASCULAR ENDOTHELIAL CROWTH FACTOR IN PLACENTA OF NORMAL TERM PRAGNANCY AND PRAGNANEY INDUCED HYPERTENSION

目的 :观察血管内皮生长因子 (VEGF)在妊高征 (PIH)胎盘中的表达情况。方法 :用免疫组织化学法 ,检测 30例正常晚孕胎盘 (对照组 )和 34例妊高征胎盘 (妊高征组 )中的VEGF表达水平 ,并作计算机图像分析 ,测定各组标本的阳性染色光度值。结果 :VEGF在正常晚孕胎盘和妊高征胎盘中均有表达 ,其分布基本一致 ,主要在滋养细胞、血管内皮及绒毛间质。计算机图像分析结果示 ,对照组VEGF阳性染色光度为 0 .2 3780±0 .0 0 434,妊高征组VEGF阳性染色光度为 0 .2 0 6 88± 0 .0 15 30 ,其中轻度 0 .2 2 6 0 3± 0 .0 0 82 8,中度 0 .2 0 36 4±0 .0 0 36 5 ,重度 0 .1915 1± 0 .0 0 44 1,妊高征组中VEGF表达明显低于对照组P <0 .0 1。且随妊高征病情的加重 ,VEGFF表达水平呈下降趋势。结论 :妊高征胎盘中VEGF表达水平降低可能与胎盘血管生成减少及胎盘滋养细胞侵入异常有关 ,在妊高征的发病中起重要作用。

Objective:To investigate the change of vascular endothelial growth factor (VEGF) in placenta of pragnancy induced hypertension (PIH).Methods:30 cases of normal term pragnancy and 34 cases of PIH were enrolled in this study.The expression of VEGF in placenta was detected by immunohistochemistry(SP).Measure the photo density of the positive stain by computer analyse system. Results:The distribution of VEGF in placenta of normal group is similar to that of the PIH group.The positive stain can be found in villous trophoblast,vessels and villous strome. The photo density of the positive stain is 0.23780±0.00434 in normal group and 0.20688±0.01530 in PIH group.The level of VEGF was significantly lower in PIH group compared with normal term pragnancy group,P<0.01.There was a trend that the level of VEGF in PIH group decreased with the severity of PIH.Conclusions:The lower VEGF expression in placenta of PIH may be related to the abnormal placenta development an play an important role in the aetiology of PIH and its complications.