健康国人肠粘膜通透性的检测

Measurement of intestinal permeability in normal Chinese volunteers

目的 :建立一项可靠、灵敏测定人尿乳果糖 (L)及甘露醇 (M)的实验方法 ,并研究健康国人口服乳果糖、甘露醇 6h后尿中排泄率的比值 (L/M)。 方法 :用高压液相色谱示差 (HPLC RI)检测法分别测定 2 0名健康志愿者口服乳果糖 10 g、甘露醇 5g测试液 ,6h后尿中的乳果糖和甘露醇浓度 ,计算乳果糖和甘露醇排泄率及其比值。 结果 :甘露醇和乳果糖 6h尿中的排泄率分别为 5 .74%± 0 .5 8% (0 .6 5 %～ 11.47% )和 0 .132 %± 0 .0 12 %(0 .0 0 5 %～ 0 .2 0 6 % ) ,L/M比值为 0 .0 2 8± 0 .0 10 (0 .0 13～ 0 .0 5 1)。 结论 :应用HPLC RI方法测量尿中乳果糖和甘露醇的含量是一种灵敏和可靠的方法 ,测量乳果糖、甘露醇排泄率的比值可反映肠粘膜通透性 ,可作为监测肠粘膜屏障功能的有效指标。

Objectives:To establish a confident and sensible method of measuring lactulose(L) and mannitol(M) in human urine, and to research lactulose and mannitol excretion ratio of urine in normal Chinese volunteers in 6 hours after taking lactulose and mannitol. Methods:20 healthy volunteers took 10g of lactulose and 5g of mannitol, and 6 hours of urine was collected. The L and M concentration was analyzed by HPLC RI. The excretion of L and M and the L/M ratio was calculated. Results:The excretion of L and M in the urine was 5.74%±0.58% (0.65 %~11.47%) and 0.132%±0.012%( 0.005 %~0.206%),while the L/M ratio was 0.028± 0.010 (0.013~0.051). Conclusions: HPLC is a kind of confident and sensible method to analyze L and M concentration. The L/M ratio can reflect the intestinal mucosal permeability and can be used to monitor intestinal barrier function in clinical practice.